miRNA Universal SYBR qPCR Master Mix is a special premix for qPCR assays based on SYBR Green I chimeric fluorescence method. The quantification of miRNA requires high specificity because the miRNA sequences are short and miRNA sequences of the same family are generally highly similar. This product contains hot-start AceTaq DNA Polymerase based on the chemical modification. With the optimized Buffer, it can greatly reduce nonspecific amplification; meanwhile, the special ROX Reference Dye makes the master mix suitable for most qPCR instruments. It only needs to add primers and templates to perform amplification reaction, without adjusting the ROX concentration on different instruments. It is recommended to use with Vazyme’s miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme #MR101).