The Single Cell Sequence Specific Amplification Kit is a one-step RT-PCR-based amplification method. It is used to achieve transcriptome amplification in single cells or trace amounts of total RNA, which is convenient to reveal the expression levels of different genes between single cells. This kit incluedes RNA extraction and purification, reverse transcription and PCR in one tube, and no additional operations are required. In this case, the kit has the advantages of saving time, reducing experimental errors, reducing pollution, and improving sensitivity. This kit is also suitable for one-step amplification of 2 – 1,000 cells, and the number of cycles of one-step amplification should be adjusted according to the number of cells.