VAHTS Universal V8 RNA-seq Library Prep Kit for MGIis specially designed for the preparation of transcriptome libraries for next generation sequencing (NGS) platforms of MGI. The kit is universal and suitable for RNA library preparation of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library preparation for non-stranded or stranded RNA-Seq transcriptome analysis. This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library preparation and shortens the operation time. The optimized reaction system improves the library preparation efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.