HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse. Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers.  4  ×  HiScript  IV  qRT  SuperMix  contains  all  the  components  required  for  the  reverse  transcription,  just  add  template  RNA  and  RNase-free ddH2O to start the reaction.